XINSHENG is a DAC column manufacturer in China, providing dynamic axial compression chromatography columns for preparative HPLC, pharmaceutical purification, process scale-up and industrial chromatographic separation. Our DAC column range covers nominal diameters from 50 mm to 1000 mm, including DAC50, DAC100, DAC150, DAC200, DAC300, DAC450, DAC800 and DAC1000 models.

Our dynamic axial compression columns use a hydraulically driven piston to compress the stationary phase during column packing and maintain axial compression during chromatographic operation. This arrangement helps preserve packed-bed stability, particularly in large-diameter preparative columns where bed settling and uneven packing can affect separation performance.

We supply DAC chromatography columns for customers establishing new purification processes, expanding existing preparative HPLC operations or replacing chromatography columns in industrial separation systems. Column specifications and supporting equipment can be reviewed according to the separation method, packing material, target processing capacity and installation requirements.

DAC Column Models and Technical Specifications

The XINSHENG DAC series includes eight listed column diameters. Model selection depends on required packed-bed volume, operating flow, pressure rating, available installation space and the stationary phase used in the purification process.

The model numbers indicate nominal column inner diameters. Column length, effective packing height, pressure rating and overall equipment dimensions vary by model. For final equipment selection, refer to the individual technical specifications.

How Dynamic Axial Compression Maintains Column Bed Stability

Large preparative chromatography columns require a packed bed with consistent density and suitable flow distribution. Inadequate packing or bed settling can create voids, uneven solvent flow and changes in chromatographic performance.

A dynamic axial compression column addresses these requirements by using an axially movable piston connected to a hydraulic compression mechanism. The piston applies controlled pressure to the stationary phase during packing and supports the packed bed during chromatography operation.

Hydraulic Compression Mechanism

The hydraulic system moves the piston through the column tube to compress the stationary phase. The appropriate compression setting depends on the column structure, packing material, column diameter and packing procedure.

Continuous Bed Support

Axial compression helps maintain contact with the packed bed as its physical condition changes during operation. This reduces the risk of bed-head voids caused by settling, while chromatographic performance remains dependent on the packing quality and operating method.

Liquid Distribution

The fluid distribution arrangement affects how the mobile phase enters and passes through the packed bed. XINSHENG's DAC800 and DAC1000 configurations use H-TREE distribution structures. Distribution components must be selected and maintained according to the column and packing media specifications.

Packing and Unpacking

The movable piston supports controlled column packing and access to the packed bed for unpacking or stationary-phase replacement. The required hydraulic sequence, tools and maintenance procedure depend on the DAC model.

DAC Column Packing, Unpacking and Maintenance

Column packing is one of the most important operating procedures for a preparative DAC system. In addition to column dimensions, buyers should evaluate how the stationary phase is loaded, compressed, inspected and eventually removed.

For users operating reusable DAC columns, access to packing instructions, replacement seals, filter plates and maintenance support should be considered during procurement. XINSHENG also offers chromatography column spare parts, including DAC50 sealing components, through its accessories range.

DAC Column Selection for Process Scale-Up

Selecting a larger DAC column is not simply a matter of increasing column diameter. The separation method, stationary phase, bed height, linear velocity, sample loading and mobile-phase consumption must be evaluated together.

Column Diameter and Flow Requirements

At a constant linear velocity, the required volumetric flow increases in proportion to the column cross-sectional area. This means a larger DAC column may require substantially greater solvent delivery capacity than the column used during method development.

For example, increasing column diameter from 100 mm to 200 mm increases the cross-sectional area by a factor of four. Maintaining the same linear velocity would therefore require approximately four times the volumetric flow, assuming comparable process conditions.

Bed Height and Packing Material

Bed height and stationary-phase particle characteristics influence column pressure and separation behavior. A process transferred to a larger DAC column should be evaluated for packing conditions, bed uniformity and the pressure limits of the selected chromatography media.

Sample Loading and Processing Capacity

Column bed volume is one factor affecting the amount of sample that can be processed, but actual loading capacity depends on compound properties, stationary-phase selectivity and required product purity. Process-scale decisions should be supported by chromatographic test results.

Supporting HPLC Equipment

Industrial DAC columns must be matched with appropriate solvent delivery pumps, sample introduction equipment, detectors, piping, valves and fraction collection arrangements. The required supporting equipment depends on the separation method and target processing capacity.

XINSHENG also supplies preparative HPLC systems and HPLC pumps for customers planning integrated chromatography equipment projects.

DAC Columns and Static Axial Compression Columns

XINSHENG's axial compression column range includes both DAC and SAC products. The two column types differ primarily in their compression mechanisms and the way the packed bed is supported during operation.

The appropriate column type depends on the chromatography process, operating conditions and packing requirements. Customers should compare actual product specifications rather than assuming that one compression method is preferable for every application.

Industrial DAC Column Applications

Dynamic axial compression columns are used in preparative chromatography processes where stable packed-bed operation and larger column diameters are required. XINSHENG's DAC product range serves separation and purification projects in pharmaceutical and related processing industries.

• Peptide Purification: Preparative chromatographic separation of peptides and peptide-related compounds using suitable stationary phases and purification methods.

• Pharmaceutical Intermediates: Isolation and purification of intermediate compounds during pharmaceutical process development and production.

• Active Pharmaceutical Ingredients: Preparative purification processes requiring appropriate column capacity, solvent compatibility and chromatographic performance.

• Natural Product Separation: Recovery and purification of target compounds from botanical extracts and other complex mixtures.

• Fine Chemical Purification: Chromatographic separation of synthetic compounds, specialty chemicals and process intermediates.

DAC450 Application in GLP-1 Production

XINSHENG has a GLP-1 production application involving a DAC-450-650 preparative HPLC column, installed in China in July 2022. This project provides a relevant reference for buyers evaluating larger-diameter chromatography columns for peptide purification.

For a comparable application, customers should provide their target compound, separation method, required processing quantity, stationary phase and purification specifications so that the appropriate column configuration can be reviewed.

Custom DAC Column Configurations and Scope of Supply

XINSHENG provides dynamic axial compression columns in multiple standard model sizes and supports discussions involving project-specific column configurations. The required equipment should be defined according to the purification process, intended installation and hydraulic operating requirements.

For large DAC column projects, the quotation should clearly define whether the hydraulic power unit, packing accessories, control equipment, installation support and spare parts are included or supplied separately.

Frequently Asked Questions

What is the difference between a DAC column and a conventional preparative chromatography column?

A DAC column uses a movable piston and hydraulic compression to establish and support the packed bed. This allows dynamic axial force to be maintained during operation. Conventional preparative columns may use other packing and bed-support arrangements, depending on their construction.

Which DAC column sizes does XINSHENG manufacture?

XINSHENG lists DAC50, DAC100, DAC150, DAC200, DAC300, DAC450, DAC800 and DAC1000 models. The nominal model numbers correspond to column inner diameters in millimeters. Technical specifications and available configurations should be confirmed for the selected size.

What is included when purchasing a DAC column?

The scope of supply should be confirmed for the selected model and project. Buyers should establish whether the quotation includes the column body, hydraulic equipment, packing accessories, control arrangements, documentation and installation services.

Can I use my existing preparative HPLC system with a new DAC column?

A new DAC column can be evaluated against the existing system's flow capacity, pressure limits, solvent compatibility, tubing connections and operating controls. Any required changes to the pump, injection equipment, detectors or supporting components should be identified before installation.

What determines the packing height of a DAC column?

Effective packing height is limited by the column design and selected model. The required packed-bed height also depends on the separation method, stationary phase and target column volume. XINSHENG's  DAC800 and DAC1000 specifications list an effective packing height of up to 450 mm.

What information should be provided for a DAC column quotation?

Please provide the required column diameter, bed height, stationary phase, mobile phase, target operating pressure, sample processing quantity and installation conditions. If the column is part of a new production project, include your purification method and any existing process data.